Journal: Cell Death & Disease

Article Title: Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC

doi: 10.1038/s41419-021-03525-x

Figure Lengend Snippet: a Diagram of experiment design of UV-radiated cSCC mouse. b Histology of representative sample of NS, AK-1/2 and cSCC from one UV- SKH-1 mouse. NS normal skin, AK actinic keratoses (AK1 and AK2 were grade 1 and grade 2 of AK), cSCC cutaneous squamous cell carcinoma.

Article Snippet: Then, XL50 cSCC cells from China Center for Type Culture Collection (Wuhan, China, 5 × 10 6 ) and NIH/3T3 cells (mouse embryonic fibroblast, 1 × 10 6 ) were injected subcutaneously into the backs of mice to establish a cSCC mouse model. After incubation, mice were injected intraperitoneally with SR1078 or DMSO solution daily.

Techniques:

Journal: Cell Death & Disease

Article Title: Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC

doi: 10.1038/s41419-021-03525-x

Figure Lengend Snippet: Tumor pathological classification and scoring of mice skin tissues.

Article Snippet: Then, XL50 cSCC cells from China Center for Type Culture Collection (Wuhan, China, 5 × 10 6 ) and NIH/3T3 cells (mouse embryonic fibroblast, 1 × 10 6 ) were injected subcutaneously into the backs of mice to establish a cSCC mouse model. After incubation, mice were injected intraperitoneally with SR1078 or DMSO solution daily.

Techniques:

Journal: Cell Death & Disease

Article Title: Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC

doi: 10.1038/s41419-021-03525-x

Figure Lengend Snippet: a Unsupervised clustering using all genes analysis showed that cSCC were significantly different compared with AK and NS. b DEGs among each comparison. ( c ) GSVA analysis among each comparison. The comparisons include AK vs. NS, cSCC vs NS, and cSCC vs. AK.

Article Snippet: Then, XL50 cSCC cells from China Center for Type Culture Collection (Wuhan, China, 5 × 10 6 ) and NIH/3T3 cells (mouse embryonic fibroblast, 1 × 10 6 ) were injected subcutaneously into the backs of mice to establish a cSCC mouse model. After incubation, mice were injected intraperitoneally with SR1078 or DMSO solution daily.

Techniques: Comparison

Journal: Cell Death & Disease

Article Title: Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC

doi: 10.1038/s41419-021-03525-x

Figure Lengend Snippet: a Distribution of NS, AK, and cSCC samples in the pseudo-time trajectory analysis. b Samples were highlighted by pseudo-time in the trajectory, which showed a progression from NS to AK then cSCC. c Co-expressed network calculated between TFs and genes among the cSCC progression-driven genes. Purple rectangle nodes represent TFs and plink elliptic nodes represent genes. d Rorα expression with regards to the trajectory pseudo-time. Rorα expression was significantly downregulated in cSCC group.

Article Snippet: Then, XL50 cSCC cells from China Center for Type Culture Collection (Wuhan, China, 5 × 10 6 ) and NIH/3T3 cells (mouse embryonic fibroblast, 1 × 10 6 ) were injected subcutaneously into the backs of mice to establish a cSCC mouse model. After incubation, mice were injected intraperitoneally with SR1078 or DMSO solution daily.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC

doi: 10.1038/s41419-021-03525-x

Figure Lengend Snippet: a Optical density of Rorα. Data were extracted from HPA database and lower optical density was obtained in cSCC. b Expression of Rorα from single-cell RNA-sequencing data. Rorα was significantly downregulated in cSCC cells than that of normal keratinocytes. c Cells were highlighted by disease state and Rorα expression level by uniform manifold approximation and projection (UMAP). d , e Representative micrographs of Rorα expression by IHC in humans. Rorα in the normal tissue showed a significantly higher expression than that in the cSCC tissue. *** p < 0.001.

Article Snippet: Then, XL50 cSCC cells from China Center for Type Culture Collection (Wuhan, China, 5 × 10 6 ) and NIH/3T3 cells (mouse embryonic fibroblast, 1 × 10 6 ) were injected subcutaneously into the backs of mice to establish a cSCC mouse model. After incubation, mice were injected intraperitoneally with SR1078 or DMSO solution daily.

Techniques: Expressing, RNA Sequencing

Journal: Cell Death & Disease

Article Title: Loss of retinoic acid receptor-related receptor alpha (Rorα) promotes the progression of UV-induced cSCC

doi: 10.1038/s41419-021-03525-x

Figure Lengend Snippet: a Representative tumors growth of SKH-1 mice after injected with cSCC cells between DMSO and SR1078 groups. b Tumor growth curve measured by tumor volume for DMSO and SR1078 groups. Tumor volumes were measured every two days and calculated as length × width 2 × 0.5 (mm 3 ). c Tumor weight at sixteen days after injection with cSCC cells. Data are presented as the means ± standard deviation. d Ki67 expression by IHC for DMSO and SR1078 groups. The proliferation of cSCC cells was attenuated by Rorα activation. * p < 0.05; ** p < 0.01, *** p < 0.001.

Article Snippet: Then, XL50 cSCC cells from China Center for Type Culture Collection (Wuhan, China, 5 × 10 6 ) and NIH/3T3 cells (mouse embryonic fibroblast, 1 × 10 6 ) were injected subcutaneously into the backs of mice to establish a cSCC mouse model. After incubation, mice were injected intraperitoneally with SR1078 or DMSO solution daily.

Techniques: Injection, Standard Deviation, Expressing, Activation Assay

Journal: Cell reports

Article Title: Single-Cell Deconvolution of Fibroblast Heterogeneity in Mouse Pulmonary Fibrosis

doi: 10.1016/j.celrep.2018.03.010

Figure Lengend Snippet: (A) Sketch of bleomycin-induced pulmonary fibrosis mouse model. (B) Workflow depicts rapid dissociation and sorting of MCs from lung tissue for generating scRNA transcriptome profiles. (C and D) 2D visualization of single-cell clustering of MC profiles inferred from RNA-seq data for all MCs in normal (C) and fibrotic (D) α SMA -GFP ;Tbx4-Cre;Rosa26-tdTomato lung samples. Six major classes of MCs in normal lung and seven major classes of MCs in fibrotic lung were detected. Endothelial cells also were included in the analysis. The percentage of each cell population was indicated. Colored bar coded as indicated. (E and F) Heat maps of MC normalized signal show MC subtypes changes by top genes (columns) for individual MC subtype cells (rows) in normal (E) and fibrotic (F) α SMA -GFP ;Tbx4-Cre;Rosa26-tdTomato lung samples. (G and H) Clustering plots depicting single-cell RNA-seq datasets for normal (G) and fibrotic (H) Tbx4-lineage + α SMA + MCs acquired from marker-based fluorescence-activated cell sorting (FACS).

Article Snippet: The Lung Gene Expression in Single-Cell (LungGENS) program separated MCs into proliferative mesenchymal progenitor, myofibroblast/smooth muscle, pericyte, intermediate fibroblast 1 and 2 and matrix fibroblast on embryonic (E) 16.5, and FB (LipoFibroblast/Matrix Fibroblast) and myofibroblast/smooth muscle on E18.5 and postnatal mouse lungs by single-cell RNA sequencing (scRNA-seq) analysis using the Fluidigm C1 platform ( ).

Techniques: RNA Sequencing, Marker, Fluorescence, FACS